Molecular Biologist (Laboratory Management) - NIH - Bethesda, MD

Company: Kelly Services
Location: Bethesda
Posted on: November 22, 2021

Job Description:

Kelly Government Solutions is a strategic supplier and business partner to the federal government and its key suppliers. Through our partnership with the National Institutes of Health, Kelly Government Solutions offers administrative, IT, engineering, scientific, and healthcare professionals the opportunity to work with the leading medical research center in the world. We are seeking an experienced individual to work as a Laboratory Manager at the National Institutes of Health in Bethesda, MD.

This is a long-term contract position which offers:

- Competitive compensation and comprehensive benefit package

+ Optional health, vision, and dental plans

-Vacation leave as well as 10 paid federal holidays and 401K plan.

- Access to NIH's unparalleled resources and niche scientific initiatives

TASKS/SERVICES. The contractor shall:

**Provides technical experience needed to assist in studying the basic principles of plant and animal life**

+ Plan and administer biological research programs

+ Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function

+ Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species

+ Develop and maintain liaisons and effective working relations with groups and individuals, agencies, and the public to encourage cooperative management strategies or to develop information and interpret findings

+ Collect, analyze and interpret data for all experiments performed

+ Search, retrieve and analyze publicly available data within the cBIOPortal for Cancer Genomics, COSMIC, dbSNP, dbVar, ClinVar, ExAC, The Human Protein Atlas, En-sembl, NCBI databases, DepMap, CCLE, GDSC, DGIdb, UniProtKBSwiss-Prot, and other online databases related to cancer, genomics, and proteomics

+ Make detailed and comprehensive records daily in laboratory notebook, to document: experimental purpose, results and interpretation thereof, and conclusions Laboratory notebook must adhere to NIH policies and lab-specific policies and will be reviewed on a regular basis by the principal investigator

+ Participate in and present research updates at weekly laboratory meetings, and on-on-one meetings with the Principal Investigator, for group and individual discussion

+ Adhere to all institutional policies, safety standards, and completion of appropriate training courses.

+ Perform PubMed and PubMed Central searches and other online searches to identify, read and assimilate scientific and biomedical literature relevant to projects; maintain a personal database of literature in EndNote.

+ Annotate gene and/or protein datasets using Ingenuity Pathway Analysis, g:Profiler, and/or DAVID.

+ Perform hierarchical clustering using Partek-- Genomics Suite--.

+ Perform and/or assist in highly accurate analysis of large-scale datasets.

+ Scan Western blots and perform densitometry, data quantification, and normalization

+ Develop X-ray films after exposure to Western blots.

+ Perform Bradford assays, Western blotting, immunoprecipitation, and co-immunoprecipitation.

+ Perform sequence alignments and sequence format conversions using bioinformatics tools including Clustal Omega, BLAST, and EMBOSS Seqret.

+ Perform macrodissection of fresh-frozen and/or paraffin-embedded human tissues and subsequent protein and nucleic acid extraction, purification, and quantification.

+ Perform protein and nucleic acid isolation and purification from mammalian cell cultures.

+ Perform molecular-based and cell-based assays including migration assays, viability assays, proliferation assays, wound-healing assays, kinase assays, reporter assays, drug sen-sitivity assays, siRNA/shRNA depletion, and in vitro ubiquitination assays.

+ Perform transient and stable transfections of plasmid DNAs into mammalian cell cultures.

+ Use BMG Omega plate reader and Bio-Rad Chemidoc Imager.

+ Perform cell counts using hemocytometer and automated cell counters including the Countess Automated Cell Counter.

+ Perform mammalian cell culture using sterile techniques; freeze stocks of mammalian cells for long-term storage and thaw stocks of frozen mammalian cells to initiate cultures.

+ Perform large-scale bacterial culture, bacterial transformation, bacterial colony growth and selection on agar plates, plasmid DNA isolation, plasmid DNA purification, and plasmid DNA quantification, and gel electrophoresis.

+ Design and generate mammalian expression constructs by site directed mutagenesis and cloning/subcloning.

+ Perform in silico predictions of sequence-variant function, using tools that include Poly-Phen-2, SIFT, Mutation Assessor, PROVEAN, SNAP2, PANTHER-PSEP, and Mut-Pred2.

+ Perform Sanger sequencing, sequence analysis, variant calling, and variant annotation in accordance with Human Genome Variation Society (HGVS) nomenclature.

+ Design and perform polymerase chain reaction (PCR) based assays and perform gel electrophoresis of PCR products.

+ Perform all research and related tasks in a meticulous manner, with scientific integrity, and including appropriate experimental controls, and following pre-approved experimental pro-tocol(s).

+ Perform tasks in a team-setting and/or individual setting, depending on project-specific needs.

**Assists with performing basic laboratory experiments and procedures**

+ Collect and analyze biological data about relationships among and between organisms and their environment

+ Supervise biological technicians and technologists and other scientists

+ Monitor and observe experiments, recording production and test data for evaluation by research personnel

+ Analyze experimental data and interpret results to write reports and summaries of findings

+ Perform all research and related tasks in a meticulous manner, with scientific integrity, and including appropriate experimental controls, and following pre-approved experi-mental protocols

+ Perform tasks in a team-setting andor individual setting, depending on project-specific needs

+ Perform PubMed and PubMed Central searches and other online searches to identify, read and assimilate scientific and biomedical literature relevant to projects; maintain a personal database of literature in EndNote

+ Ship, store, and dispose of biological and hazardous materials according to NIH guide-lines and policies

+ Assist with Western blotting

+ Assist with data analysis

+ Assist with manuscript preparation including preparation of data, tables, and figures.

+ Assist with basic laboratory experiments and procedures

**Prepares, organizes and maintains various lab samples, supplies, and equipment**

+ Operate, calibrate, or maintain equipment used in quantitative or qualitative analysis, such as spectrophotometers, calorimeters, flame photometers, or computer-controlled analyzers

+ Maintain laboratory equipment

+ Operate laboratory equipment to analyze samples

+ Analyze laboratory findings

+ Perform Sanger sequencing, sequence analysis, variant calling, and variant annotation in accordance with Human Genome Variation Society HGVS nomenclature

+ Perform in silico predictions of sequence-variant function using tools that include Poly-Phen-2, SIFT, Mutation Assessor, PROVEAN, SNAP2, PANTHER-PSEP, and Mut-Pred2

+ Perform large-scale bacterial culture, bacterial transformation, bacterial colony growth and selection on agar plates, plasmid DNA isolation, plasmid DNA purification, and plasmid DNA quantification, and gel electrophoresis

+ Perform mammalian cell culture using sterile techniques and freeze stocks of mammalian cells for long-term storage

+ Maintain and organize liquid nitrogen and freezer inventories

+ Perform cell counts using hemocytometer and automated cell counters including the Countess Automated Cell Counter

+ Perform transient and stable transfections of plasmid DNAs into mammalian cell cultures

+ Perform molecular-based and cell-based assays including migration assays, viability as-says, proliferation assays, wound-healing assays, kinase assays, siRNAshRNA depletion, and in vitro ubiquitination assays

+ Perform protein and nucleic acid isolation and purification from mammalian cell cul-tures

+ Perform microdissection of fresh-frozen andor paraffin-embedded human tissues and subsequent protein and nucleic acid extraction, purification, and quantification

+ Perform sequence alignments and sequence format conversions using bioinformatics tools including Clustal Omega, BLAST, and EMBOSS Seqret

+ Perform Bradford assays, Western blotting and immunoprecipitation

+ Perform andor assist in highly accurate analysis of large-scale datasets

+ Perform hierarchical clustering using Partek Genomics Suite

+ Annotate gene andor protein datasets using Ingenuity Pathway Analysis, g: Profiler, andor DAVID

+ Ensures lab complies with green lab program initiatives; may also help complete the green lab assessment if other point-of-contact not available.

+ Establish and maintain laboratory protocols, including SOPs.

+ Communicate in a timely, effective, and civil manner with NHGRI staff, service providers, and other internal and external personnel/stakeholders.

+ Monitor freezers daily for proper temperature; schedule service and/or repair of freezers that are out of normal temperature range; relocate contents of freezer to another location if freezer temperature is out of range
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Keywords: Kelly Services, Bethesda , Molecular Biologist (Laboratory Management) - NIH - Bethesda, MD, Healthcare , Bethesda, Maryland