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Scientist

Company: 22nd Century Technologies Inc.
Location: Bethesda
Posted on: June 19, 2022

Job Description:

Job Title: ScientistLocation: Bethesda MD 21224Duration: 5 years Overall Position Summary and Objectives Investigate the pathways of genome metabolism involving helicase enzymes defective in hereditary disorders displaying age-associated clinical features and/or cancer. Understand the molecular and cellular roles of human DNA helicases in pathways that are important for the maintenance of genome integrity and cellular homeostasis. Genetic and biochemical approaches are used to characterize the roles of helicases in cellular nucleic acid metabolism through their catalytic functions and protein interactions. Use of siRNA/CRISPR screens, pharmacologically active small molecules, single-molecule DNA fiber studies, live cell imaging by immunofluorescence confocal microscopy, protein biochemistry, and enzyme kinetic assays.Minimum Education

  • Master'sCertifications & Licenses
    • Minimum Seven (3-5) years of experience in Molecular Biology, Protein Biochemistry, or a related field.
    • PhD degree in Biological Sciences or other related discipline. Three (3) years of specialized experience plus a Master's degree is equivalent to a Ph.D. Five (5) years of specialized experience plus a BA/BS degree is equivalent to a Ph.D.Skills (Ranked by Priority) --- Knowledge of proper laboratory disposal methods and safety procedures.--- Ability to work independently and think critically with skilled hands and sound judgment.--- Strong communications skills, both oral and written.--- Excellent analytical, organizational and time management skills.--- Experience with isolation of DNA and RNA from tissues and cells, small samples, PCR, immunohistochemistry, Western blotting, current methodologies of gene targeting and analysis; RNA interference, transfections, and prior experience working with cell models.--- Familiarity with flow cytometric techniques. --- Familiarity with tissue culture techniques.--- Strong familiarity with cell biology, genetics, state-of-the-art molecular biology and its techniques.--- In-depth knowledge of single-cell DNA replication and DNA repair assays and single-molecule (kinetic) biochemical assays.Software
      • MS Office Field of Study
        • Genetics
        • Biology
        • Molecular BiologyDeliverables
          • Work products and documents related to performing investigations with reagents (material-al/tissues) under investigation which range from cellular products to whole tissue; maintain inventory system and sample storage; maintain inventory of all experiments.
          • Work products and documents related to performing molecular biology experimentations, bioinformatics, gene and protein profiling, and protein concentration in extracts; safeguard animal logs; maintain and breed specific mouse models.
          • Work products and documents related to carrying out routine tasks; follow strict methodologies in carrying out analyses and demonstrated practical procedures.
          • Work products and documents related to preparing specimens and samples; maintain and operate standard laboratory equipment; present ongoing research and findings at weekly meetings.
          • Work products and documents related to writing and editing scientific papers; create and maintain a research tracking system and database for all research activities, daily records of experiments and results, including research documentation.Provides technical experience needed to assist in studying the basic principles of plant and animal life
            • Perform mathematical calculations for the preparation of graphs
            • Write and edit scientific papers for submission to peer-reviewed journals
            • Present ongoing research and findings at weekly lab unit section meetings
            • Present papers abstract posters at scientific meetings conferences
            • Design and synthesize nucleic acid substrates for biochemical assays to analyze proteins involved in nucleic acid metabolism
            • Assists with performing basic laboratory experiments and procedures
              • Perform biochemical assays including electrophoretic mobility shift assay EMSA, helicase assays with radiometric or fluorometric substrates, ATPase assays, protein interaction assays ELISA, co-immunoprecipitation, dot blot, affinity pull-down
              • Work with staff on the maintenance of animal protocols; work with animal facility staff to maintain, design and produce the necessary crosses of distinct mouse strains Safeguard animal logs; work with animal facility staff to maintain and breed specific mouse models
              • Work with staff on the maintenance of laboratory protocols; work with facility staff to maintain, design and produce the necessary safety protocols and procedures
              • Perform molecular biology experimentations, bioinformatics, gene and protein profiling, protein concentrations in extractsPrepares, organizes and maintains various lab samples, supplies, and equipment
                • Work with staff on scheduling and maintenance of research tracking system and databases for all research activities, daily records of experiments and results, including research documentation
                • Create and maintain a research tracking system and database for all research activities, daily records of experiments and results, including research documentation
                • Operate laboratory equipment to analyze samples

Keywords: 22nd Century Technologies Inc., Bethesda , Scientist, Other , Bethesda, Maryland

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